LabKate said:One practical note: write down what you did and when, before you need it.
This is exactly what I could not find anywhere else. Sending this to two other people who asked me the same thing last week.
LabKate said:One practical note: write down what you did and when, before you need it.
This is exactly what I could not find anywhere else. Sending this to two other people who asked me the same thing last week.
Clinical perspective, offered as context rather than as advice. Order of operations matters more than any single choice here: establish a baseline, change one thing, wait long enough for it to express itself, then measure again under the same conditions.
If somebody has the primary source to hand I would rather cite it than paraphrase it.
james_edin said:Order of operations matters more than any single choice here: establish a baseline, change one thing, wait long enough for it to express itself, then…
This is my experience too, for whatever a second data point is worth. The detail I would add is minor and it is already implied above.
Trusted third-party HPLC & mass spectrometry analysis. Verify peptide purity with the lab the community relies on. Independent. Accurate. Transparent.
Verify Your PeptidesEst. 1998. The synthesis house behind the vials you send for testing. ISO 9001 and cGMP certified, 1,500+ staff, batch-specific COA with every order.
Browse GL Biochemjames_edin said:Order of operations matters more than any single choice here: establish a baseline, change one thing, wait long enough for it to express itself, then…
Coming at james_edin’s question from a different direction. The version of this that has an answer is narrower than the version being asked. Narrow it and it becomes tractable; leave it broad and the thread will produce nine confident and incompatible replies.
OP back with an update, since a thread like this is useless without one.
Closing this out. What actually changed my mind was the point about waiting for a trend instead of reading a single measurement, which is embarrassing in hindsight and worth saying anyway.