Good breakdown. Let me add something about
retention time because this is how identity is confirmed.
Every compound has a characteristic retention time on a given HPLC column under specific conditions. Janoshik runs reference standards alongside your sample. If your sample's main peak elutes at the same time (±0.2 min typically) as the reference standard, that confirms identity.
> If the purity is 98% but the retention time doesn't match the reference standard,
you have a very pure sample of the wrong compound.
This has happened. I've seen a result where someone sent in "semaglutide" and it came back 96% pure — but the retention time matched BPC-157, not semaglutide. The vendor had mislabeled the vials. Without the identity confirmation, you'd think everything was fine.
Always check the identity match, not just the purity number.
Last edited: May 3, 2026 at 7:39 PM