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Evidence-based GLP-1 & peptide discussion since 2023
ForumsCOA & Analytical TestingInter-lab variability in peptide testing — July 2024 Page 4

Inter-lab variability in peptide testing — July 2024

sean_dublin Mon, Feb 23, 2026 at 12:36 PM 30 replies 1,343 viewsPage 4 of 6
chris_chi24
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Mar 10, 2026 at 9:33 AM#16

Adding the clinical framing, because it changes how the question reads.

Third-party testing services for certificates of analysis besides Janoshik:

  • Janoshik: Gold standard for peptide community, ~$129, 10-14 day turnaround
  • Valitech: US-based, ISO 17025 accredited, ~$249
  • Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
  • ChemTox: Basic identity and purity testing, ~$119

Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.

Last edited: Mar 10, 2026 at 12:33 PM
16 14nick_newbie, DadBodDave, AmyNC_wife and 13 others
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A1cHero_PHX
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Mar 12, 2026 at 6:11 AM#17
anders_CPH said:
Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in…

Vendor quality matters when discussing certificates of analysis. I've tested my last 3 batches through Janoshik and purity has ranged from 98.4% to 99.0%. Consistency is key.

For anyone new: always request the COA, and consider independent verification. The $300 for a Janoshik test is worth the peace of mind.

17 15KarenAZ_mom, zoe_NC, Dr.ObesityLA and 14 others
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NurseAsh_DET
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Mar 14, 2026 at 2:47 AM#18
anders_CPH said:
Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in…

This is exactly what I could not find anywhere else. Adding it to my notes with a link back to this thread.

18 16BrianDallas92, labquiet_amy, emily_PDX and 15 others
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Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

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sophie_paris
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Mar 15, 2026 at 11:22 PM#19

Clinical perspective, offered as context rather than as advice.

My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed:

Main peak retention time: 14.2 minutes (consistent with semaglutide reference standard)
Peak purity: 98.6%
Related substances: 0.6% (single impurity at 8.6 minutes — likely a synthesis intermediate)
Mass spec: m/z 4113.58 (matches expected molecular weight)

This is a clean result. The mass spec confirmation is particularly important — it proves the molecule is actually semaglutide and not a substitution.

19 17AussieAnna, BethLabQueen, ChrisMacros and 16 others
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mike_mod
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Mar 17, 2026 at 7:57 PM#20

Moderator note: good thread. Keeping it here rather than moving it, because the question is general enough to be useful. No action needed from anybody.

41 14PurityPaulOR, MaxMetOK, MounjBrad and 38 others
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