🍪 CompoundTalk uses cookies to improve your experience, analyze traffic, and personalize content. By continuing to use this site, you agree to our Cookie Policy.
Evidence-based GLP-1 & peptide discussion since 2023
ForumsCOA & Analytical TestingUnderstanding peptide purity percentages — what worked for you? Page 4

Understanding peptide purity percentages — what worked for you?

Dr.NateNeph Tue, Nov 11, 2025 at 4:06 PM 29 replies 1,285 viewsPage 4 of 6
SleepDoc_PDX
Member
289
1,234
Sep 2024
Portland, OR
Nov 15, 2025 at 9:00 PM#16
JenPlateau said:
My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed: Main peak retention time:…

Adding a me-too, because a thread of one person's experience is not much use. The detail I would add is minor and it is already implied above.

37 12pete_nash, hank_denver, carlos_SATX and 34 others
Reply Quote Save Share Report
Dr.RaviCardio
VIP Member
2,890
15,678
Jan 2024
New York, NY
Nov 16, 2025 at 2:20 AM#17
anders_CPH said:
My vendor review relevant to certificates of analysis: ordered 6 times from my current compounder.

Vendor quality matters when discussing certificates of analysis. I've tested my last 5 batches through Janoshik and purity has ranged from 98.0% to 99.2%. Consistency is key.

For anyone new: always request the COA, and consider independent verification. The $300 for a Janoshik test is worth the peace of mind.

Last edited: Nov 16, 2025 at 3:20 AM
36 11anna.melb_AU, mark_tokyo, hans_munich and 33 others
Reply Quote Save Share Report
SandraNC_45
Member
312
1,345
Sep 2024
Charlotte, NC
Nov 16, 2025 at 7:40 AM#18

One thing that is still open after anders_CPH’s answer:

Did your prescriber agree with that reading, and if not what was their objection?

35 10NicoleRaleigh, james_edin, FranDenver and 32 others
Reply Quote Save Share Report

Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

Shop Reference Standards
lisa_labSD
Member
278
1,234
Oct 2024
San Diego, CA
Nov 16, 2025 at 1:00 PM#19
Dr.RaviCardio said:
Vendor quality matters when discussing certificates of analysis.

Community-verified vendors for certificates of analysis: I maintain a spreadsheet of Janoshik-verified test results submitted by community members. Across 46 submitted tests:

  • 82% tested at ≥98% purity
  • 12% tested at 95-98% purity
  • 6% tested at <95% purity (concerning)

The vendors with consistently high purity results are listed in the Vendor Directory. We update monthly based on new community submissions.

Last edited: Nov 16, 2025 at 4:00 PM
34 9lori_vegas, Dr.PulmRoch, maya_sedona and 31 others
Reply Quote Save Share Report
Admin
Administrator
2,456
9,812
Oct 2023
Online
Nov 16, 2025 at 6:20 PM#20

Moderator note: reminder that nothing in this thread is medical advice, and that clinical claims need a source. No action needed from anybody.

12 12PeptideSynthNJ, Dr.KarenChen, Dr.NateNeph and 9 others
Reply Quote Save Share Report

Similar Threads

HPLC vs UPLC for peptide purity — method comparison study18 replies
Mass spectrometry for peptide identity verification — ESI-MS guide7 replies
Endotoxin testing methods — LAL vs recombinant Factor C17 replies
Red flags on COAs — how to spot a fake certificate5 replies
USP reference standards for peptide verification — sourcing guide3 replies
ForumsNewTrendingMembersAccount

Log In

Forgot password?
No account? Register