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ForumsCOA & Analytical TestingInter-lab variability in peptide testing — July 2024 Page 4

Inter-lab variability in peptide testing — July 2024

Dr.GastroMayo Fri, Sep 19, 2025 at 1:04 AM 41 replies 1,493 viewsPage 4 of 9
DeniseRN_TPA
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Oct 3, 2025 at 3:15 PM#16

Clinical perspective, offered as context rather than as advice.

I tested my certificates of analysis batch through Janoshik — 99.2% purity. That's within acceptable range for compounded peptides. Turnaround was 10 business days.

The test costs ~$146 but gives you actual HPLC chromatography data plus mass spec confirmation. For the peace of mind alone, it's worth every penny. I test every new batch from a new vendor.

37 12jim_asheville, matt_MKE, Dr.ReproEndo and 34 others
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GenomicsKate
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Oct 2024
Cambridge, MA
Oct 5, 2025 at 9:29 AM#17
DeniseRN_TPA said:
I tested my certificates of analysis batch through Janoshik — 99.2% purity.

Vendor quality matters when discussing certificates of analysis. I've tested my last 3 batches through Janoshik and purity has ranged from 98.4% to 99.4%. Consistency is key.

For anyone new: always request the COA, and consider independent verification. The $300 for a Janoshik test is worth the peace of mind.

36 11KristenIndy, MarkLI_maint, Dr.PeteFamMed and 33 others
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jennifer_SEA
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Seattle, WA
Oct 7, 2025 at 3:42 AM#18
DeniseRN_TPA said:
I tested my certificates of analysis batch through Janoshik — 99.2% purity.

COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis:

  • No HPLC data: If there's no chromatography graph, it's not a real COA
  • Exact 100% purity: Suspicious — real testing always shows slight variation
  • No batch number: Can't trace back to specific production
  • No endotoxin testing: For injectables, this is essential
  • Template looks like it was made in Word: Professional labs have standardized formats

I have seen fake COAs that are literally photoshopped. Always verify independently if possible.

35 10matt_MKE, Dr.ReproEndo, lucas_SP_BR and 32 others
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Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

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DebRD_ATL
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Feb 2024
Atlanta, GA
Oct 8, 2025 at 9:54 PM#19
DeniseRN_TPA said:
I tested my certificates of analysis batch through Janoshik — 99.2% purity.

This answered a question I did not know how to ask. Printing the relevant bit and taking it with me.

34 9PedsEndoPhilly, SleepDoc_PDX, RegAffairsDC and 31 others
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mike_mod
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Oct 10, 2025 at 4:05 PM#20

Moderator note: a post naming a supplier has been edited. Discuss suppliers in the review sections, not here. Carry on.

Last edited: Oct 10, 2025 at 6:05 PM
12 12PurityPaulOR, MaxMetOK, MounjBrad and 9 others
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