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Evidence-based GLP-1 & peptide discussion since 2023
ForumsCOA & Analytical TestingHas anyone dealt with moisture content analysis for lyophilized peptides? Page 3

Has anyone dealt with moisture content analysis for lyophilized peptides?

NauseaFreeNow Sun, Mar 16, 2025 at 5:34 AM 42 replies 1,836 viewsPage 3 of 9
mike.trainer_LA
Senior Member
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Apr 2024
Los Angeles, CA
Mar 17, 2025 at 8:27 PM#11
rachel_ABQ said:
HPLC results interpretation for certificates of analysis: when you get your Janoshik report back, here's what the numbers mean: Purity %: Should be…

My vendor review relevant to certificates of analysis: ordered 5 times from my current compounder. Consistency has been excellent.

Shipping: Same-day local pickup
Packaging: Professional, discreet, proper labeling with lot/batch numbers
Purity: 98.6% average across orders (Janoshik verified)
Customer service: Responsive within 6 hours
Price: $150/month for tirzepatide 10mg equivalent

27 0Dr.AddMedPHL, newstart_MO, mia_MS2 and 24 others
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wanda_boise
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Aug 2024
Boise, ID
Mar 18, 2025 at 7:33 PM#12
Dr.PeteFamMed said:
Agreed, and mass spec answers a question purity does not: identity.

This is exactly what I could not find anywhere else.

28 1marco_milano, pam_columbus, nick_SD_fit and 25 others
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KevinCompounds
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Dec 2023
Nevada
Mar 19, 2025 at 6:39 PM#13
rachel_ABQ said:
HPLC results interpretation for certificates of analysis: when you get your Janoshik report back, here's what the numbers mean: Purity %: Should be…

Third-party testing services for certificates of analysis besides Janoshik:

  • Janoshik: Gold standard for peptide community, ~$139, 10-14 day turnaround
  • Valitech: US-based, ISO 17025 accredited, ~$249
  • Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
  • ChemTox: Basic identity and purity testing, ~$119

Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.

Last edited: Mar 19, 2025 at 8:39 PM
29 2MikeKY_noInsulin, Dr.RaviCardio, jennifer_SEA and 26 others
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Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

Shop Reference Standards
anders_CPH
Senior Member
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Feb 2024
Copenhagen, DK
Mar 20, 2025 at 5:46 PM#14
NauseaFreeNow said:
Two reports on the same product from two laboratories, four percentage points apart, and no method stated on either.

Community-verified vendors for certificates of analysis: I maintain a spreadsheet of Janoshik-verified test results submitted by community members. Across 58 submitted tests:

  • 82% tested at ≥98% purity
  • 12% tested at 95-98% purity
  • 6% tested at <95% purity (concerning)

The vendors with consistently high purity results are listed in the Vendor Directory. We update monthly based on new community submissions.

30 3BethLabQueen, ChrisMacros, KetoKyle and 27 others
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mike_mod
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Nov 2023
New York
Online
Mar 21, 2025 at 4:53 PM#15

Moderator note: reminder that nothing in this thread is medical advice, and that clinical claims need a source.

Last edited: Mar 21, 2025 at 6:53 PM
31 4PurityPaulOR, MaxMetOK, MounjBrad and 28 others
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