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ForumsCOA & Analytical TestingWhat even IS a COA and why should I care — my results so far Page 3

What even IS a COA and why should I care — my results so far

RunnerRach Thu, Jan 9, 2025 at 11:36 AM 13 replies 1,672 viewsPage 3 of 3
mel_PDX
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Jan 10, 2025 at 10:38 PM#11
claudia_zurich said:
They are not contradictory, they measure different things, and this is the single most common misreading of a certificate.

That reframing is the part I needed.

11 9Dr.NateNeph, PharmD_Rodriguez, julia.endo and 8 others
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LibrarianMeg
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Jan 11, 2025 at 8:13 AM#12

Adding the clinical framing, because it changes how the question reads.

Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in the refrigerator.

Day 1: 100.1% purity
Day 14: 99.1% purity
Day 28: 99.5% purity

Minimal degradation over 28 days when properly refrigerated. This confirms the standard 28-day beyond-use date is appropriate. Beyond 28 days, I'd reconstitute a fresh vial to be safe.

12 10PharmD_Rodriguez, julia.endo, JessicaM_2024 and 9 others
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Dr.RenalNash
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Jan 11, 2025 at 5:47 PM#13
Dr.RaviCardio said:
I tested my certificates of analysis batch through Janoshik — 98.4% purity.

Third-party testing services for certificates of analysis besides Janoshik:

  • Janoshik: Gold standard for peptide community, ~$126, 10-14 day turnaround
  • Valitech: US-based, ISO 17025 accredited, ~$206
  • Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
  • ChemTox: Basic identity and purity testing, ~$116

Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.

13 11Dr.NutriCornell, pam_stl, wei_SG and 10 others
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Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

Shop Reference Standards
jason_paloalto
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Palo Alto, CA
Jan 12, 2025 at 3:21 AM#14
RunnerRach said:
The provenance questions are more useful than the numbers.

COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis:

  • No HPLC data: If there's no chromatography graph, it's not a real COA
  • Exact 100% purity: Suspicious — real testing always shows slight variation
  • No batch number: Can't trace back to specific production
  • No endotoxin testing: For injectables, this is essential
  • Template looks like it was made in Word: Professional labs have standardized formats

I have seen fake COAs that are literally photoshopped. Always verify independently if possible.

14 12andrew_nyc, Dr.EndoEP, GraceAZ_72 and 11 others
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Admin
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Jan 12, 2025 at 12:55 PM#15

Moderator note: a couple of off-topic posts removed.

15 13PeptideSynthNJ, Dr.KarenChen, Dr.NateNeph and 12 others
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