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Evidence-based GLP-1 & peptide discussion since 2023
ForumsCOA & Analytical TestingUnderstanding peptide purity percentages — what worked for you? Page 4

Understanding peptide purity percentages — what worked for you?

andrew_nyc Tue, Jul 9, 2024 at 12:22 PM 25 replies 2,441 viewsPage 4 of 5
zoe_NC
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Nov 2024
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Jul 12, 2024 at 7:31 PM#16
Dr.PainCLE said:
Vendor quality matters when discussing certificates of analysis.

Can confirm. Same sequence, different timescale. Posting only so the count is not one.

50 23Dr.BariatricHTX, LindaRN_retired, tommy_boulder and 47 others
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Dr.NateNeph
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Jul 13, 2024 at 4:57 AM#17

Adding the clinical framing, because it changes how the question reads.

Third-party testing services for certificates of analysis besides Janoshik:

  • Janoshik: Gold standard for peptide community, ~$125, 10-14 day turnaround
  • Valitech: US-based, ISO 17025 accredited, ~$205
  • Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
  • ChemTox: Basic identity and purity testing, ~$105

Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.

1 24NauseaFreeNow
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tommy_boulder
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Jul 13, 2024 at 2:23 PM#18
Dr.NateNeph said:
Third-party testing services for certificates of analysis besides Janoshik: Janoshik: Gold standard for peptide community, ~$125, 10-14 day turnaround…

Adding a me-too, because a thread of one person's experience is not much use. Nothing to add that would improve it.

Last edited: Jul 13, 2024 at 3:23 PM
2 0robert_kc, dan_philly
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Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

Shop Reference Standards
nancy_portland
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Aug 2024
Portland, ME
Jul 13, 2024 at 11:49 PM#19
Dr.NateNeph said:
Third-party testing services for certificates of analysis besides Janoshik: Janoshik: Gold standard for peptide community, ~$125, 10-14 day turnaround…

COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis:

  • No HPLC data: If there's no chromatography graph, it's not a real COA
  • Exact 100% purity: Suspicious — real testing always shows slight variation
  • No batch number: Can't trace back to specific production
  • No endotoxin testing: For injectables, this is essential
  • Template looks like it was made in Word: Professional labs have standardized formats

I have seen fake COAs that are literally photoshopped. Always verify independently if possible.

3 1Dr.PeteFamMed, claudia_zurich, nancy_portland
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Admin
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Jul 14, 2024 at 9:15 AM#20

Moderator note: leaving this open. It is being argued well and the disagreement is the useful part.

25 23PeptideSynthNJ, Dr.KarenChen, Dr.NateNeph and 22 others
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