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ForumsCOA & Analytical TestingLC-MS/MS vs HPLC for peptide analysis — November 2025 Page 4

LC-MS/MS vs HPLC for peptide analysis — November 2025

TrialTracker_MD Sat, Apr 26, 2025 at 1:49 PM 39 replies 2,208 viewsPage 4 of 8
Dr.PathRoch
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Apr 29, 2025 at 10:56 AM#16

Adding the clinical framing, because it changes how the question reads.

COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis:

  • No HPLC data: If there's no chromatography graph, it's not a real COA
  • Exact 100% purity: Suspicious — real testing always shows slight variation
  • No batch number: Can't trace back to specific production
  • No endotoxin testing: For injectables, this is essential
  • Template looks like it was made in Word: Professional labs have standardized formats

I have seen fake COAs that are literally photoshopped. Always verify independently if possible.

44 17MikeFit_NJ, InsuranceTom, WendyG_ATL and 41 others
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Dr.NateNeph
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Apr 29, 2025 at 6:34 PM#17
Dr.PulmRoch said:
Vendor quality matters when discussing certificates of analysis.

Third-party testing services for certificates of analysis besides Janoshik:

  • Janoshik: Gold standard for peptide community, ~$129, 10-14 day turnaround
  • Valitech: US-based, ISO 17025 accredited, ~$229
  • Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
  • ChemTox: Basic identity and purity testing, ~$119

Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.

45 18NauseaFreeNow, SteveThurs, B12Beth and 42 others
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PharmacoVig_BOS
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Feb 2024
Boston, MA
Apr 30, 2025 at 2:12 AM#18
Dr.PulmRoch said:
Vendor quality matters when discussing certificates of analysis.

Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in the refrigerator.

Day 1: 100.1% purity
Day 14: 99.1% purity
Day 28: 98.1% purity

Minimal degradation over 28 days when properly refrigerated. This confirms the standard 28-day beyond-use date is appropriate. Beyond 28 days, I'd reconstitute a fresh vial to be safe.

Last edited: Apr 30, 2025 at 5:12 AM
46 19DataDave, Dr.GutHealth, amsterdam_pete and 43 others
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Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

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TirzTom
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Apr 30, 2025 at 9:50 AM#19

A narrower follow-up, since the general answer is now clear:

Was that from a primary source or from a summary of one?

47 20SandraNC_45, Dr.EndoIndy, tom_AK and 44 others
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Admin
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Apr 30, 2025 at 5:28 PM#20

Moderator note: a post naming a supplier has been edited. Discuss suppliers in the review sections, not here.

19 17PeptideSynthNJ, Dr.KarenChen, Dr.NateNeph and 16 others
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