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ForumsCOA & Analytical TestingLC-MS/MS vs HPLC for peptide analysis — November 2025 Page 6

LC-MS/MS vs HPLC for peptide analysis — November 2025

TrialTracker_MD Sat, Apr 26, 2025 at 1:49 PM 39 replies 2,208 viewsPage 6 of 8
Dr.PathRoch
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May 6, 2025 at 2:42 PM#26

The figures, for anyone assembling their own picture. If you are comparing against somebody else’s result, check that you are comparing the same measurement taken the same way. Most of the apparent contradictions in these threads dissolve at that step.

That is the short version; the long version is somebody else's post.

25 23MikeFit_NJ, InsuranceTom, WendyG_ATL and 22 others
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nick_newbie
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May 7, 2025 at 5:24 PM#27

A narrower follow-up, since the general answer is now clear:

How long did you give it before you decided it was working?

26 24dan_philly, MeganSA_TX, LarryQC_SD and 23 others
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Dr.EndoEP
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Oct 2024
El Paso, TX
May 8, 2025 at 8:06 PM#28
Dr.PathRoch said:
If you are comparing against somebody else’s result, check that you are comparing the same measurement taken the same way.

This is where I part company with the consensus forming above. Filing an objection on the n=1 problem. Personal experience is genuine evidence about one person and very weak evidence about anybody else, and this thread keeps promoting the first into the second.

Correct me if the detail matters more than I have assumed.

Last edited: May 8, 2025 at 10:06 PM
27 0FranDenver, Dr.BariatricHTX, LindaRN_retired and 24 others
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TinaHashiRN
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Sep 2024
Raleigh, NC
May 9, 2025 at 10:47 PM#29
Dr.PathRoch said:
If you are comparing against somebody else’s result, check that you are comparing the same measurement taken the same way.

Correct, and worth adding that it is reversible. Nothing being described here is a one-way door, which changes how cautious it is reasonable to be.

28 1Admin, Dr.Martinez, mike_mod and 25 others
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TrialTracker_MD
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Jan 2024
Maryland
May 11, 2025 at 1:27 AM#30

Reporting back.

Update: the thing I was blaming turned out not to be the cause. Leaving the original post as written rather than editing it, so the mistake stays visible for whoever searches this next.

50 23lori_vegas, Dr.PulmRoch, maya_sedona and 47 others
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