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ForumsCOA & Analytical TestingLC-MS/MS vs HPLC for peptide analysis — November 2025 Page 10

LC-MS/MS vs HPLC for peptide analysis — November 2025

Dr.SportsMedIN Mon, Jul 22, 2024 at 3:37 PM 51 replies 2,260 viewsPage 10 of 11
lisa_labSD
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Jan 25, 2025 at 4:30 AM#46

Adding the numbers, since they settle part of this. One practical note: write down what you did and when, before you need it. Reconstructing a timeline from memory three months later is how people end up unable to answer the one question that would have resolved it.

Happy to go further on any of that.

9 7stefan_berlin, Dr.EM_Chicago, pete_RVA and 6 others
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Dr.SportsMedIN
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Jan 31, 2025 at 8:07 PM#47

Reporting back.

Reporting back for the archive rather than for the thread: I did the narrow version of what was suggested, gave it the full interval, and it settled. Posting it because threads without a follow-up are half a thread.

10 8lisa_labSD, adam_van, Dr.SurgeonPGH and 7 others
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carlos_SATX
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San Antonio, TX
Feb 7, 2025 at 11:41 AM#48
Dr.SportsMedIN said:
Reporting back for the archive rather than for the thread: I did the narrow version of what was suggested, gave it the full interval, and it settled.

Worth saying that the confident version of this is more useful to the person writing it than to the person reading it.

Last edited: Feb 7, 2025 at 1:41 PM
11 9Dr.BariatricHTX, LindaRN_retired, tommy_boulder and 8 others
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Dr.ReproEndo
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Feb 14, 2025 at 3:11 AM#49
Dr.SportsMedIN said:
Reporting back for the archive rather than for the thread: I did the narrow version of what was suggested, gave it the full interval, and it settled.

Dr.SportsMedIN has the substance of this right. The condition it depends on is worth stating. Yes, and the corollary is less popular: if the reasoning holds, it also holds where it produces an answer we do not like.

12 10GraceAZ_72, carl_compliance, DanielChem_CHI and 9 others
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KarenAZ_mom
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Feb 20, 2025 at 6:37 PM#50

Old thread, but it comes up first when you search for this, so it is worth updating. The boring version of this is the one that works, and the boring version is: measure a baseline, change one variable, wait, measure again under the same conditions. Nobody wants that answer and it is still the answer.

34 7HPLC_Greg, LibrarianMeg, bri_stats and 31 others
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