Adding the numbers, since they settle part of this. One practical note: write down what you did and when, before you need it. Reconstructing a timeline from memory three months later is how people end up unable to answer the one question that would have resolved it.
Ask again with the specifics and you will get a better answer than this one.
One thing that is still open after anders_CPH’s answer:
What would you measure differently if you were starting again?
BiostatsBrad said:One practical note: write down what you did and when, before you need it.
Filing a mild objection. Mild because I might be wrong; an objection because nobody has addressed the case that does not fit. This treats a plausible mechanism as a demonstrated one. Plausible is where you start looking, not where you stop.
Trusted third-party HPLC & mass spectrometry analysis. Verify peptide purity with the lab the community relies on. Independent. Accurate. Transparent.
Verify Your PeptidesEst. 1998. The synthesis house behind the vials you send for testing. ISO 9001 and cGMP certified, 1,500+ staff, batch-specific COA with every order.
Browse GL BiochemBiostatsBrad said:One practical note: write down what you did and when, before you need it.
True, with the qualification that this is a self-selected group. The people for whom it did not work post less, and that shapes everything we think we know.
BiostatsBrad said:One practical note: write down what you did and when, before you need it.
This answered a question I did not know how to ask. Sending this to two other people who asked me the same thing last week.